The effects of diethyl pyrocarbonate on the stability and activity of plant polyribosomes.
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چکیده
منابع مشابه
Polyribosome isolation in the presence of diethyl pyrocarbonate.
Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however ...
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Profiles of polyribosomes were obtained from etiolated stem segments of Pisum sativum L. var. Alaska isolated in various buffers. Tissue homogenized in a medium containing 0.2 m tris-HCl, pH 8.5, 0.2 m sucrose, 30 mm MgCl(2), and 60 mm KCl yielded polyribosomes exhibiting far less degradation than tissue homogenized in conventional media containing tris-HCl at lower ionic strength and pH. A fur...
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Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however ...
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Profiles of polyribosomes were obtained from etiolated stem segments of Pisum sativum L. var. Alaska isolated in various buffers. Tissue homogenized in a medium containing 0.2 M tris-HCI, pH 8.5, 0.2 M sucrose, 30 mM MgCL, and 60 mM KCI yielded polyribosomes exhibiting far less degradation than tissue homogenized in conventional media containing tris-HCl at lower ionic strength and pH. A furthe...
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where [E]/[E], is fractional enzyme activity, and t is reaction time. The constants C and k were determined graphically (Defares & Sneddon, 1960). The results of the effect of diethyl pyrocarbonate on DEAE-cellulose column-chromatography fractions obtained from two samples of normal gastric mucosa and three samples of stomach adenocarcinoma are presented in summary form in Table I. It will be s...
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ورودعنوان ژورنال:
- Plant physiology
دوره 48 6 شماره
صفحات -
تاریخ انتشار 1971